![]() The dermal layer includes a porous fiber matrix with cross-linked bovine tendon collagen and glycosaminoglycan (chondroitin-6-sulfate) ( Burke, 1984 Burke, 1987 Burke et al., 1981 Yannas & Burke, 1980). Integra ® artificial skin is a bi-layered structure consisting a dermal replacement layer covered with a silicone sheet as an outer layer. The biomaterials used for supporting skin cell growth include natural biodegradable polymers such as collagen and gelatin, as well as synthetic biodegradable polymers such as α-polyester and poly(ε-caprolactone) (PCL) ( Hajiali et al., 2011 Ng, Khor & Hutmacher, 2004).įor the demand of the durability, elasticity, cosmetic appearance of normal skin and wound repair, several functional dermal layers were developed. The concept of design for the tissue engineered skin equivalent is based on existing models comprising a stratified epithelium grown on a matrix populated with dermal fibroblasts ( El-Ghalbzouri et al., 2002). By designing and incorporating specific therapeutic factors in skin substitutes, the promotion of wound healing as well as reduction of morbidity and mortality for large wounds may be achieved. Highly innovative tissue-engineered skin substitutes have been developed to mimic normal skin recently with melanocytes, a capillary-like network, sensory innervation and adipose tissue ( Bechetoille et al., 2007 Regnier et al., 1997 Tremblay et al., 2005 Trottier et al., 2008). Collagen-based wound dressings have been applied in the treatment of burn and ulcer patients over the last 30 years ( Doillon & Silver, 1986 Peters, 1980). Collagen based materials are the most well-known biocomposites in clinical use. Therefore, biocomposite materials are often used in contact with living tissues such as scaffolds for cell-based therapy, biomedical implants and controlled drug delivery devices. Generally, biocomposite materials have better structural properties than either constituent material alone. This study may provide an effective and low-cost wound dressings to assist skin regeneration for clinical use.īiocomposites, biocompatible and/or eco-friendly composites, can be formed by different varieties of natural and synthetic polymers including polysaccharides, proteins and biodegradable synthetic polymers. According to our data, a significant promotion in wound healing and skin regeneration could be observed in GC LP H seeded with adipose-derived stem cells by Gomori’s trichrome staining. Due to reasonable mechanical strength and biocompatibility in vitro, G/C/P with a lower content of collagen and a higher content of PCL (GC LP H) was selected for animal wound healing studies. G/C/P biocomposites with a lower collagen content showed better cell proliferation than those with a higher collagen content in vitro. ![]() All G/C/P biocomposites exhibited similar cell growth and mechanical characteristics in comparison with C/P biocomposites. Good cytocompatibility was present in all G/C/P biocomposites when incubated with primary human epidermal keratinocytes (PHEK), human dermal fibroblasts (PHDF) and human adipose-derived stem cells (ASCs) in vitro. Scanning electron microscopy showed that all biocomposites had similar fibrous structures. Resultsĭifferential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 ☌. Two different GC:PCL ratios (1:8 and 1:20) were used. G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. PeerJ 7: e6358 Ī tissue-engineered skin substitute, based on gelatin (“G”), collagen (“C”), and poly(ε-caprolactone) (PCL “P”), was developed. A gelatin/collagen/polycaprolactone scaffold for skin regeneration. Cite this article Wei L, Chang H, Wang Y, Hsu S, Dai L, Fu K, Dai N. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. ![]() Licence This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. DOI 10.7717/peerj.6358 Published Accepted Received Academic Editor Wei-Chun Chin Subject Areas Bioengineering, Biotechnology, Cell Biology, Dermatology Keywords Gelatin, Skin tissue engineering, Poly(ε-caprolactone)(PCL), Collagen, Adipose-derived stem cells Copyright © 2019 Wei et al. 7 Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.
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